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Director: d William C. Davis
Contact
Flow cytometric methods are useful for characterizing properties
of individual cells, quantifying the distribution of cells that
bear a specific phenotype within a mixed population of cells,
and sorting specific types of cells. Flow cytometry is also used
in cell cycle analysis and determination of chromosome ploidy
in animals and plants.
For assistance with cell sorting, multi-parametric phenotypic and functional
analyses of cells, contact
Bill Davis
509-335-6051 or davisw@wsu.edu
For assistance with experimental design, technical support or data analysis,
CRB members may contact either Dr. Davis.
Services and Location
Located in Animal Disease and Biotechnology Facility (ADBF
Rm 4075), the WSU College of Veterinary Medicine operates this
facility. The facility has three flow cytometers: FACScan, FACSort,
FACSVantage (Becton Dickinson Immunocytometry Systems) and two
Macintosh workstations for data analysis. The capabilities of
these three machines are summarized below.
The FACScan is a simple flow cytometer. It has a single laser
and can analyze five separate parameters (side and forward scatter
light scatter and 3 fluorescent markers).
The FACSort has two lasers and can analyze and sort cells based
on 6 separate parameters (side and forward scatter, and 4 fluorescent
markers).
The FACS Vantage SE is a high-speed cell sorter. It has two lasers
and can analyze and sort based on 8 separate parameters (side
and forward scatter and 6 fluorescent markers).
All flow cytometers have internet access and are equipped with
software for data acquisition and analysis.
There are two separate computer workstations for data analysis.
Technical Support and Training
Technical support is available to users of this facility.
The FACScan and FACSort are user-friendly flow cytometers
that can be used by students, staff, and faculty without technical
assistance after learning how to operate them. Technical and
operations support will be provided to CRB members who wish to
use these machines. Training will be provided to CRB members
who will require more than occasional use of these machines.
The FACS Vantage requires technical assistance. Arrangements
have to be made to schedule use. Training in the use of the sorter
will be provided to a designated technician or graduate student
in laboratories requiring long-term heavy use of the sorter.
Access and Fees
Authorized individuals have access to the core facility at
all times during the week and weekends. Frequent users can obtain
key badges for entrance into the flow cytometry room. A key to
the room will be available in Dr. Davis' laboratory for occasional
users of the facility, Bustad Hall Rm 324 (Mary Jo Hamilton will
show authorized users where the key is kept). Users need to reserve
time they will be using the flow cytometers. Call Dr. Davis'
lab, 5-6058, to reserve time on the flow cytometers.
Current Flow Cytometry Core Facility users are an hourly fee
for the use of the machines. The current rate for CRB members
is $30/hr (the rate is $60/hr for non-CRB members). There is
no charge for the use of computers for data analysis.
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