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Director: Lisa Shaffer
Contact: 509-368-6710
Summary:
This core provides (1) routine karyotype analysis on the investigator's
established human cell lines or human clinical samples; (2) molecular
cytogenetic analysis (fluorescent in situ hybridization, FISH)
using the investigator's clone and either the investigator's
cell lines or clinical samples, or on normal control cell lines
from the Core; (3) 24-color karyotyping of human cell lines to
identify complex rearrangements; (4) mapping of human large-insert
clones from the investigator's laboratory to determine a map
position in normal control cell lines from the Core; (5) assist
in identifying human large-insert clones for use in the Core;
(6) lymphoblastic EBV transformation of human peripheral blood
samples to establish permanent cell lines; (7) store investigator's
established cell lines, provide growing cultures back to investigators,
and provide fixed cell pellets back to investigators. The Core
is willing to engage in karyotype analysis of non-human cell
lines and clinical specimens if investigators are willing to
supply normal examples from several individuals from a species
in order for the Core to gain expertise prior to performing karyotypic
analysis on clinical specimens. Future work could extend into
FISH on non-human cell lines using clones and cell lines provided
from the investigators.
Services/Resources:
Routine karyotype analysis of human cell lines
FISH analysis of investigator-provided clones
24-color FISH analysis to identify complex karyotypes in investigator-provided
cell lines
FISH mapping of large-insert clones or identification of clones
from the Human Genome Project
Lymphoblast transformation of human peripheral blood samples
using EBV
Storage of investigator's cell lines
Tissue culture expansion of investigator's cell lines
Collaboration on non-human cell lines for karyotype analysis
or FISH
Zeiss Axioplan fluorescent microscope equipped with filters
to view rhodamine, fluorescein, and DAPI
Applied Imaging image capture and analysis system for G-banded
karyotypes and FISH
Color printer suitable for publication
Queue 140C freezer for storage of cell lines
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