Associate Professor Department of Vet Micro & Path. Washington State University Pullman, Wa 99164-7040 Phone: 509-335-6313 Fax: 509-335-8529 Email:drcall@wsu.edu Faculty Web Page   Ph.D. 1997, Washington State University, Zoology RESEARCH INTERESTS: Fish Reproduction RESEARCH SUMMARY: My research program emphasizes the use of microarray technologies for molecular epidemiology, diagnostics and genetics of emerging pathogens. This work includes development of salmonid diagnostics in conjunction with the WSU and UofI Salmon Restoration Program. I have additional interests in environmental sampling of pathogens, evolution of antibiotic resistance in food animals, comparative genomics of food-borne pathogens, and immunology.   REPRESENTATIVE PUBLICATIONS: Call, DR, FJ Brockman, and DP Chandler. 2001. Detecting and genotyping Escherichia coli O157:H7 using multiplexed PCR and nucleic acid microarrays. International Journal of Food Microbiology 67:71-80. Loge, FJ, DE Thompson, and DR Call. 2002. PCR detection of specific pathogens in water: A risk-based analysis. Environmental Science and Technology 35:2754-2759. Call, DR, MK Borucki and FJ Loge. 2003. Detection of bacterial pathogens in environmental samples using DNA microarrays. Journal of Microbiological Methods 53:235-243. Call, DR, MK Borucki, and TE Besser. 2003. Mixed-genome microarrays reveal multiple serotype and lineage-specific differences for Listeria monocytogenes. Journal of Clinical Microbiology 41:632-639. Call, DR, MK Bakko, MJ Krug, and MC Roberts. 2003. Identifying antimicrobial resistance genes using DNA microarrays. Antimicrobial Agents and Chemotherapy 47:3290-3295. See related article by Holzman, D. 2003. Microarray analyses may speed antibiotic resistance testing. ASM News 69 (Nov):538-539. González, SF, MJ Krug, ME Nielson, Y. Santos, and DR Call. 2004. Simultaneous detection of marine fish pathogens using multiplexed PCR and DNA microarrays. Journal of Clinical Microbiology 42:1414-1419. Khachatryan, AR, DD Hancock, TE Besser, and DR Call. 2004. The role of calf-adapted Escherichia coli in maintenance of antibiotic resistance in dairy calves. Applied and Environmental Microbiology 70:752-757. Lane, S., J. Evermann, F. Loge and D.R. Call. 2004. Secondary structure prevents target hybridization to oligonucleotide microarrays. Biosensors and Bioelectronics 20:728-735. Warsen, A, MJ Krug, S LaFrentz, DR Stanek, FJ Loge, and DR Call. 2004. Simultaneous discrimination between 15 fish pathogens using 16S rDNA PCR and DNA microarrays. Applied and Environmental Microbiology 70:4216-4221. Borucki, MK, J Reynolds, DR Call, T Ward, B Page, and J Kadushin. 2005. Suspension arrays for direct and high throughput subtyping of Listeria monocytogenes from genomic DNA. Journal of Clinical Microbiology 43:3255-3259. Broschat, SL, FJ Loge, JD Peppin, D White, DR Call, and E Kuhn. In press. An optical reflectance assay for the detection of biofilm formation. Journal of Biomedical Optics. Call, DR. 2005. Challenges and opportunities for pathogen detection using DNA microarrays. Critical Reviews in Microbiology 31:91-99. Soule, M, K. Cain, S. LaFrentz, and DR Call. 2005. Combining suppression subtractive hybridization and microarrays to map the intraspecies phylogeny of Flavobacterium psychrophilum. Infection and Immunity 73:3799-3802. Soule, M, S LaFrentz, K Cain, S LaPatra, and DR Call. 2005. Polymorphisms in 16s rRNA genes of Flavobacterium psychrophilum correlate with elastin hydrolysis and tetracycline resistance. Diseases of Aquatic Organisms 65:209-216. Call, DR, MS Kang, J Daniels, and TE Besser. 2006. Assessing genetic diversity in plasmids from Escherichia coli and Salmonella enterica using a mixed-plasmid microarray. Journal of Applied Microbiology. 100:15-28.