Professor of Zoology & Interim Chair Department of Biological Sciences University of Idaho Aquaculture Program University of Idaho Moscow, ID 83844-3051   Phone: 208-885-6388 FAX: 208-885-7905 E-mail: jcloud@uidaho.edu    Ph.D., 1974 University of Wisconsin-Madison RESEARCH INTERESTS: Fish Reproduction RESEARCH SUMMARY: The overall objective of our research program is directed toward understanding germ cell development in teleosts. The projects that are ongoing in my laboratory involve the reproduction and early development of rainbow trout; these projects include gonadal regeneration following surgical ablation of both gonads, phenotypic sex reversal, ovarian development in triploid females, rescue of haploid embryos by transfer of diploid cells into the blastula, and sperm cryopreservation. Dr. Cloud is holding a canister from a liquid nitrogen tank containing straws of frozen Chinook Salmon sperm that was collected by the Nez Pierce Tribe. The University of Idaho, Department of Biological Sciences, have a cooperative project with the Nez Pierce Tribe to freeze and hold the Chinook Salmon sperm.   REPRESENTATIVE PUBLICATIONS: Bouma GJ, Cloud JG, Nagler JJ. 2005 An in vitro system for the long-term tissue culture of juvenile rainbow trout (Oncorhynchus mykiss) testis. J Exp Zoolog A Comp Exp Biol. 303(8):698-703. Cloud, JG. 2003. Surgical transplantation of sexually immature ovaries of rainbow trout (Oncorhynchus mykiss). J Exp Zool 298:73-76. Ingermann RL, Holcomb M, Robinson ML, Cloud JG. 2002 Carbon dioxide and pH affect sperm motility of white sturgeon (Acipenser transmontanus). J Exp Biol 205(Pt 18):2885-90. Bencic, D.C., R. L. Ingermann and J. G. Cloud. 2001. Does CO2 enhance short-term storage success of chinook salmon (Oncorhynchus tshawytscha) milt? Theriogenology 56:157-166. Nagler, J.J., J.G. Cloud, P.A. Wheeler, and G.H. Thorgaard. 2001. Testis transplantation in male rainbow trout (Oncorhynchus mykiss). Biology of Reproduction 64:644-646. Bencic, D.C., J. G. Cloud and R. L. Ingermann. 2000. Carbon dioxide reversibly inhibits sperm motility and fertilizing ability in steelhead trout (Oncorhynchus mykiss). Fish Physiology and Biochemistry 23:275-281. Krisfalusi, M., P.A. Wheeler, G.H. Thorgaard and J.G. Cloud. 2000. Gonadal morphology of female diploid gynogenetic and triploid rainbow trout. Journal of Experimental Zoology 286:505-512. Bencic, D. C., M. Krisfalusi, J.G. Cloud and R.L. Ingermann. 2000. Short-term storage of salmonid sperm in air versus oxygen. North American Journal of Aquaculture 62: 19-25. Wendling, N. C., D.C. Bencic, J.J. Nagler, J.G. Cloud, R.L. Ingermann. 2000. Adenosine triphosphate levels in chinook salmon (Oncorhynchus tshawytscha) eggs following in vitro maintenance and activation/fertilization. Fish Physiology and Biochemistry 22:217-223. Cloud, J. G. 2000. Post-thaw fertility of salmonid sperm relative to time of freezing after collection. In "Cryopreservation in Aquatic Species", Advances in World Aquaculture, Vol 7 (T.R. Tiersch and P.M. Mazik, eds) Published by the World Aquaculture Society. Pp. 101-103. Cloud, J.G., R. Armstrong, P.A. Wheeler, P.A. Kucera and G.H. Thorgaard. 2000. Northwest salmonid germplasm repository: A model for genetic conservation. In "Cryopreservation in Aquatic Species", Advances in World Aquaculture, Vol 7 (T.R. Tiersch and P.M. Mazik, eds). Published by the World Aquaculture Society. Pp. 338-342. Nagler, J.J., J.E. Parsons and J.G. Cloud. 2000. A single pair mating scheme indicates maternal effects on embryo survival in rainbow trout (Oncorhynchus mykiss). Aquaculture 184:177-183. Krisfalusi, M. and J.G. Cloud. 1999. Gonadal sex reversal in triploid rainbow trout (Oncorhynchus mykiss). Journal of Experimental Zoology 284:466-472. Bencic, D. C., M. Krisfalusi, J.G. Cloud and R.L. Ingermann. 1999. ATP levels of chinook salmon (Oncorhynchus tshawytscha) sperm following in vitro exposure to various oxygen tensions. Fish Physiology and Biochemistry 20:389-397.