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RESEARCH INTERESTS: Oviduct Physiology RESEARCH SUMMARY: In our lab, we are currently looking at human male factor infertility. Studies into sperm and oviduct (Fallopian or uterine tube) interactions provide valuable insight into diagnostic and therapeutic techniques for use in treating male factor infertility of animals and men. Furthermore, abnormal interaction of sperm with oviduct cells in an in vitro system can be used to identify exposure of the male to toxicants and or pharmaceuticals which influence sperm function. Sperm may be able to participate in fertilization of an egg, and yet may contain significant DNA alterations such that normal pregnancy outcome does not occur. Initially, we have done studies to evaluate and better describe how sperm are stored in the woman's oviduct. This is an area that has been poorly understood. We are able to observe changes in sperm chromatin stability during this storage which may, in part, be a cause of abnormal reproduction outcome.
REPRESENTATIVE PUBLICATIONS: Ellington, J.E., Evenson D., Rice G., Brisbois S., Hiss, G., Jones, A., Broder, S., & Wright R. Coculture of human sperm and bovine oviduct epithelial cells decreases sperm chromatin structural changes seen over time in culture. Fertility and Sterility 69:643-649 (1998). Ellington, J.E. , Jones, A.E., Davitt, C.M., Schneider, C.S., Brisbois, R.S., Hiss, G.A., & Wright, R.W. Human sperm function in coculture with human, macaque, or bovine oviduct epithelial cell monolayers. Human Reproduction 13:2797-2804 (1998). Goldman, E., Ellington, J.E., Farrell, P.B. & Foote, R.H. Reaction of fresh and frozen bull spermatozoa incubated with fresh and frozen bovine oviduct epithelial cells. Reprod Dev and Nutr 38:281-288 (1998). Ellington, J.E., Evenson D.P., Brisbois R.S., Hiss, G.A., Jones, A.E., & Wright R.W. The higher quality human sperm in a sample selectively attach to oviduct (Fallopian tube) epithelial cells in vitro. Fertility and Sterility, 71:924-929 (1999). Ellington, J.E., Samper, J.C., Jones, A.E., Oliver, S.A., Burnett, K.M., & Wright, R.W. Effects of bovine serum albumin on cryopreserved stallion sperm function during media culture and oviduct epithelial cell coculture. Amer Jour of Vet Research, 60:363-367 (1999). Ellington, J.E., Broemeling, L.D., Broder, S.J., Jones, A.E., Choker, D.A., Wright, R.W. Comparison of fresh and cryopreserved human sperm attachment to bovine oviduct (uterine tube) epithelial cells in vitro. Jour of Andrology, 20:492-499 (1999). Ellington, J.E., Samper, J.C., Jones, A.E., Oliver, S.A., Burnett, K.M., & Wright, R.W. In vitro interactions of cryopreserved stallion spermatozoa and oviduct (uterine tube) epithelial cells or their secretory products. Animal Reproduction Science, 56:51-65 (1999). Schneider, C., Ellington, J.E., & Wright, R. Effects of bulls with different field fertility on in vitro cleavage and development using sperm coculture systems. Theriogenology 51:1085-1098 (1999). Ellington, J.E., Davitt CM, Smetana LD, Schneider CS, Oliver S.A, Broder S, Wright R.W.Interactions between human sperm and Fallopian tube (oviductal) cells in vivo and in vitro. Human Repro, Submitted (1999). Bosch P, de Avila JM, Ellington JE, Wright RW Jr. (2001)
Heparin and Ca2+-free medium can enhance release of bull sperm
attached to oviductal epithelial cell monolayers. |
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