B. Paige Lawrence Associate Professor Department of Pharmaceutical Sciences Washington State University Pullman, Wa 99164-6534 Phone: 509-335-1691 Fax: 509-335-5902 Email:bpl@wsu.edu   Ph.D. 1993, Cornell University, Cell Biology   RESEARCH INTERESTS: Reproductive and Developmental Toxicology/Immunology and Immunotoxicology RESEARCH SUMMARY: My laboratory is interested in determining how exposure to pollutants affect complex biological systems. Specific focus is currently on dioxins and the cellular and molecular mechanisms by which these pollutants impair the immune response to influenza virus infection. In addition to examining how exposure to dioxin alters a mature immune system, we also study how prenatal and lactational exposure to dioxin impairs the development of a functional immune system. The relationship of this research and reproductive biology is that, in addition to being a potent immunosuppressant, 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD or dioxin) is a known toxicant to the reproductive system. As with TCDD's immunotoxic effects, the cellular and molecular mechanisms that underlie the reproductive and developmental toxicities resulting from exposure to TCDD remain largely unknown. In addition to mechanistic studies of TCDD immunotoxicity, my laboratory has recently started pursuing studies to further characterize several adverse effects of TCDD on the developing fetus and reproductive tissue.   REPRESENTATIVE PUBLICATIONS: B.P. LAWRENCE, T.K. Warren, and H. Luong. (2000) Fewer T lymphocytes and decreased pulmonary influenza virus burden in mice exposed to 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD). J. Toxicol. Environ. Health, Part A. 61, 101-115. T.K. Warren, K.A. Mitchell, and B.P LAWRENCE. (2000) Exposure to 2,3,7,8-tetrachlorodibenzo-p-dioxin suppresses the cell-mediated and humoral immune response to influenza A virus without affecting cytolytic activity in the lung. Toxicological Sci. 56, 114-123. B.P. LAWRENCE, Y. Will, D.J Reed, and N.I. Kerkvliet.(2000) g-Glutamyltranspeptidase knockout mice as a model for understanding the consequences of diminished glutathione on T cell-dependent immune responses. Eur. J. Immunol. 30 (7), 1902-1910. K.K. Mann, R.A. Matulka, M.E. Hahn, A.F. Trombino, B.P. LAWRENCE, N.I. Kerkvliet, and D.H. Sherr. (1999) The role of polycyclic aromatic hydrocarbon metabolism in dimethylbenz[a]anthracene-induced pre-B lymphocyte apoptosis. Toxicol. Appl. Pharmacol. 161(1), 10-22. B.P. LAWRENCE, M. Meyer, D.J Reed, and N.I Kerkvliet. (1999) Role of glutathione and reactive oxygen intermediates in 2,3,7,8-tetrachlorodibenzo-p-dioxin-induced immune suppression in C57Bl/6 mice. Toxicological Sci. 52, 50-60. B.P. LAWRENCE and N.I. Kerkvliet. (1998) Role of altered arachidonic acid metabolism in 2,3,7,8-tetrachlorodibenzo-p-dioxin-induced immune suppression in C57Bl/6 mice. Toxicological Sci. 42(1), 13-22. C.A. Lee, B.P. LAWRENCE, N.I. Kerkvliet, and A.B. Rifkind (1998). 2,3,7,8-Tetrachlorodibenzo-p-dioxin induction of cytochrome P450-dependent arachidonic acid metabolism in mouse liver microsomes: Evidence for species-specific differences in responses. Toxicol. Appl. Pharmacol. 153, 1-11. B.P. LAWRENCE, M. Leid and N.I. Kerkvliet. (1996) Distribution and behavior of the Ah receptor from murine T lymphocytes. Toxicol. Appl. Pharmacol. 138, 275-284. B.P. LAWRENCE and W.J. Brown (1993) Inhibition of protein synthesis separates autophagic sequestration from the delivery of lysosomal enzymes. J. Cell Sci. 105, 473-480 B.P. LAWRENCE and W.J. Brown (1992) Autophagic vacuoles rapidly fuse with preexisting lysosomes in cultured hepatocytes. J. Cell Sci. 102, 515-526