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RESEARCH INTERESTS: Spermatogenesis, spermatogonial stem cells, Sertoli cells. RESEARCH SUMMARY: My lab investigates the regulation of sperm production in mice, bulls
and pigs. We investigate the biological activity of spermatogonial stem
cells and the regulation of Sertoli cells in the testis. Spermatogonial
stem cells are an adult stem cell population in the testis that provides
differentiated cells for the continuous production of sperm in adult males
while also maintaining a stem cell population. We investigate the factors
that regulate spermatogonial stem cell biological activity in vivo and
in vitro. The approach allows us to answer basic questions regarding the
differentiation and self-renewal of these unique cells. Sertoli cells
are the somatic cells that support germ cell differentiation in the testis
and are essential for sperm production. We are interested in determining
how the Sertoli cell population is established in the testis and how this
effects sperm production in adult animals. This research has impacts in
the basic understanding of reproduction in model species such as mice
and reproductive efficiency in livestock.
REPRESENTATIVE PUBLICATIONS: McLean, DJ. 2005. Spermatogonial stem cell transplantation and testicular
function. Cell Tissue Res. 322(1):21-31.
McLean DJ, Friel PJ, Pouchnik DJ, Griswold MD. 2002. Oligonucleotide microarray ananlysis of gene expression in FSH treated rat Sertoli cells. Mol Endo, in press. McLean DJ, Russell LD, Griswold MD. 2002. Biological Activity and Enrichment of Spermatogonial Stem Cells in VAD and Hyperthermia Exposed Testes from Mice Based on Colonization Following Germ Cell Transplantation. Biol Reprod, 66:1374-1379. McLean DJ, Johnston DS, Griswold MD. Characterization of spermatogonial stem cell maturation and differentiation in neonatal mice. In preparation. Oatley JM, de Avila DM, McLean DJ, Griswold MD, Reeves JJ. 2002. Transplantation of bovine germinal cells into mouse testes. J Anim Sci. 2002 Jul;80(7):1925-31. Kroft TL, Jethanandani P, McLean DJ, Goldberg E. 2001. Methylation of CpG dinucleotides alters binding and silences testis-specific transcription directed by the mouse lactate dehydrogenase promoter. Biol Reprod, 65:1522-1527. Komada M, McLean DJ, Griswold MD, Russell LD, Soriano P. 2000. E-MAP-115, encoding a microtubule-associated protein, is a retinoic acid-inducible gene required for spermatogenesis. Genes Dev 14(11):1332-42 McLean DJ, Jones LG, Jr, Froman DP. 1997. Reduced glucose transport in sperm from roosters (Gallus domesticus) with heritable subfertility. Biol Reprod, 57:791-795. McLean DJ, Feltmann AJ, Froman DP. 1998. Transfer of sperm into a chemically defined environment by centrifugation through 12% (wt/vol) Accudenz®. Poultry Sci, 77:163-168. McLean DJ, Froman DP. 1996. Identification of a sperm cell attribute responsible for subfertility of roosters homozygous for the rose comb allele. Biol Reprod, 54:168-172. McLean DJ, Korn N, Perez BS, Thurston RJ. 1993. Isolation and characterization of mitochondria from turkey spermatozoa. J Androl, 14:433-438. Froman DP, McLean DJ. 1996. Objective measurement of sperm motility based on sperm penetration of Accudenz®. Poult Sci, 75:776-784. Froman DP, Feltmann AJ, McLean DJ. 1997. Increased fecundity resulting from semen donor selection based upon in vitro sperm motility. Poult Sci, 76:73-77. |
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