Edward E. Schmidt Associate Professor Department of Veterinary Molecular Biology Montana State University Bozeman, MT 59717 Phone: 406-994-6375 Fax: 406-994-4303 Email:eschmidt@montana.edu   Ph.D. Oregon State University, Corvallis, Oregon 1990 - Biochemistry & Biophysics   RESEARCH INTERESTS: Gene Regulation RESEARCH SUMMARY: My primary research interests are to understand the intricate gene regulatory mechanisms that function in development and maintenance of complex organisms, like ourselves. Much of our work involves analyses of mouse lines we produce that bear targeted mutations (e.g., “knockouts”); however, our approaches to understanding the roles of the mutated genes are broad, including phylogenetics, genetics, biochemistry, molecular biology, histology, biophysics, genomics, proteomics, and other techniques. The biological processes we are studying include early embryonic patterning and development, placental physiology, germ cell maturation, immune cell functions, and the maternal/fetal immune interaction.   REPRESENTATIVE PUBLICATIONS: Schmidt, E. E., E. S. Hanson, and M. R. Capecchi (1999) Sequence-independent assembly of mRNAs into mRNP particles in spermatids. Mol. Cell. Biol. 19;3904-3915. Schmidt, E. E., D. S. Taylor, J. R. Prigge, S. Barnett, and M. R. Capecchi (2000) Illegitimate Cre-dependent chromosome rearrangements in transgenic mouse spermatids. Proc. Natl. Acad. Sci USA, 97;13702- 13707. Sealey, A.L., N.K. Hobbs, and E.E. Schmidt (2002) Molecular genotyping of the mouse scid allele. J. Imm. Meth. 260;303-304. Hobbs, N.K., A.A. Bondareva, S. Barnett, M.R. Capecchi, and E.E. Schmidt (2002) Removing the vertebratespecific TBP N terminus disrupts placental b2m-dependent interactions with the maternal immune system. Cell 110, 43-54. Schmidt, E.E., Bondareva, A.A., Radke, J.R., and Capecchi, M.R. (2003) Fundamental cellular processes do not require verterbrate-specific sequences within the TATA-binding protein, TBP. J. Biol. Chem. 278, 6168-6174. Bondareva, A.A. and E.E. Schmidt (2003). Early vertebrate evolution of the TATA-binding protein. Mol. Biol. Evol. 20, 1932-1939. Tucker, T.A., J.A. Kundert, A.A. Bondareva, and E.E. Schmidt (2005). Reproductive and Neurological Quakingviable (Qkv) Phenotypes in a Severe Combined Immune Deficient (SCID) Mouse Background. Immunogenetics (in press). Schmidt, E.E. (2006). The origins of polypeptide domains. Submitted, in review. Prigge, J.R., and E.E. Schmidt (2006). Interaction of protein inhibitor of activated STAT (PIAS) proteins with the TATA-binding protein, TBP. J. Biol. Chem., in press.