Microbiology Team Leader Northwest Fisheries Science Center National Marine Fisheries Service 2725 Montlake Blvd. East Seattle, WA 98112-2097 Phone: 206-860-3377 Fax: 206-860-3467 Email:mark.strom@noaa.gov   Ph.D. 1992, University of Washington, Microbiology RESEARCH INTERESTS: Salmon Infectious Disease RESEARCH SUMMARY: Basic and Applied Studies on the genetics of pathogenesis and host resistance mechanisms of the salmonid pathogen Renibacterium salmoninarum; mechanisms of host colonization, protein secretion, and virulence of Aeromonas salmonicida, A. hydrophila, and Vibrio vulnificus; rapid, molecular-based diagnostic methods for fish pathogens, alternative therapeutic treatment for bacterial kidney disease in salmon Disease Diagnostic Support Fish pathology and disease diagnostics in support of NWFSC/NMFS captive broodstock and marine fish aquaculture programs   REPRESENTATIVE PUBLICATIONS: Fairgrieve, W. T., C. L. Masada, M. E. Peterson, W. C. McAuley, G. C. McDowell, and M. S. Strom. 2006. Concentrations of erythromycin and azithromycin in mature Chinook salmon (Oncorhynchus tshawytscha) after intraperitoneal injection, and in their progeny. Dis. Aquat. Org. 68:227-234. Paranjpye, R. N. and M. S. Strom. 2005. A Vibrio vulnificus type IV pilin contributes to biofilm formation, adherence to epithelial cells and virulence. Infect. Immun. 73:1411-1422. Fairgrieve, W.T., C.L. Masada, W.C. McAuley, M.E. Peterson, M.S. Myers and M.S. Strom. 2005. A comparative evaluation of accumulation and clearance of orally administered erythromycin and its derivative, azithromycin, in juvenile fall chinook salmon (Oncorhynchus tshawytscha). Dis. Aquat. Org. 64:99-106. Rhodes, L. D., C. K. Rathbone, S. C. Corbett, L. W. Harrell, and M. S. Strom. 2004. Efficacy of cellular vaccines and genetic adjuvants against bacterial kidney disease in Chinook salmon (Oncorhynchus tshawytscha). Fish & Shellfish Immunology 16:461 Waknitz, F. W., R. N. Iwamoto, and M. S. Strom. 2003. Interactions of Atlantic salmon in the Pacific Northwest. IV. Impacts on the local ecosystems. Fisheries Research (In Press). Moore, M. M. and M. S. Strom. 2003. Infection and mortality by the yeast Metschnikowia bicuspidata var. bicuspidata in Chinook salmon fed live adult California brine shrimp (Artemia franciscana). Aquaculture (In Press). Nilsson, W. B., R. N. Paranjpye, A. DePaola, and M. S. Strom. 2003 Sequence heterogeneity in the 16S rRNA gene of Vibrio vulnificus as a possible indicator of strain virulence. J. Clin. Microbiol. 41:442-446. Rhodes, L. D., A. Coady, and M. S. Strom. 2002. Expression of duplicate msa genes in the salmonid pathogen Renibacterium salmoninarum. Appl. Environ. Microbiol. 68:5480-5487. Masada, C. L., S. E. LaPatra, A. W. Morton, and M. S. Strom. 2002. An Aeromonas salmonicida type IV pilin is required for virulence in rainbow trout (Oncorhynchus mykiss). Dis. Aquat. Org. 51:13-25. Nilsson, W. B. and M. S. Strom. 2002. Detection and identification of bacterial pathogens of fish in kidney tissue using terminal restriction fragment length polymorphism (T-RFLP) analysis of 16s rRNA genes. Dis. Aquat. Org. 48:175-185. Strom, M. S. and S. L. Lory. 2001. Type IV Prepilin Leader Peptidases. In The Enzymes", 3rd Edition, Volume 22, "Co- and Posttranslational Proteolysis of Proteins, R. E. Dalbey and D. S. Sigman (ed), Academic Press, p. 127-159. Strom, M. S. and R. N. Paranjpye. 2001. Characterization of components of type IV pili and type II extracellular secretion in Vibrio vulnificus and determination of their role in virulence. Bull. Natl. Res. Int. Aquacult. Suppl. 5:35-44. Kirov, S. M., T. C. Barnett, C. M. Pepe, M. S. Strom, and M. J. Albert. 2000. Investigation of the role of the type IV Aeromonas pilus (Tap) in the pathogenesis of Aeromonas gastrointestinal infection. Infect. Immun. 68:4040-4048. Rhodes, L. D., T. H. Grayson, S. M. Alexander, and M. S. Strom. 2000. Description and characterization of IS994, a putative IS3 family insertion sequence from the salmon pathogen, Renibacterium salmoninarum. Gene 244:97-107. Strom, M. S. and R. N. Paranjpye. 2000. Epidemiology and pathogenesis of Vibrio vulnificus. Microbes and Infection 2: 177-188. O'Farrell, C. L. and M. S. Strom. 1999. Differential expression of the virulence-associated protein p57 and characterization of its duplicated gene msa in virulent and attenuated strains of Renibacterium salmoninarum. Dis. Aquat. Org. 38:115-123. Paranjpye, R. N., J. C. Lara, J. C. Pepe, C. M. Pepe and M. S. Strom. 1998. The type IV leader peptidase-N-methyltransferase of Vibrio vulnificus controls factors required for type II protein secretion, adherence to epithelial cells, and virulence in iron-overloaded mice. Infect. Immun. 66:5659-5668