Professor & Director School of Biological Sciences Washington State University Pullman, WA 99164-4236   Phone: 509-335-7438 FAX: 509-335-3184 E-mail: thorglab@wsu.edu    Ph.D., 1977 University of Washington RESEARCH INTERESTS: Fish Genetics and Reproduction RESEARCH SUMMARY: Research in our laboratory involves genetic studies in trout and salmon, with special emphasis on rainbow trout. We apply the chromosome set manipulation techniques of gynogenesis and androgenesis to produce clonal lines. Clonal lines of rainbow trout are being used for genetic mapping and with the goal of identifying major genes affecting adaptive and economically important traits. By analyzing inheritance of DNA markers and traits of interest in controlled matings, we can assess where major genes are located on the trout genetic map. Another of our interests is the genetic basis of sex determination in trout and salmon. We are collaborating with researchers who are using the clonal lines for reproductive biology, immunology and physiological research. Studies using DNA markers and chromosome polymorphisms are also being used to assess relationships among salmonid populations to address conservation concerns. Paul Wheeler, a Research Technologist in Dr. Thorgaard's laboratory, is holding an experimental Rainbow Trout. This laboratory investigates genetics and reproduction in Trout and Salmon.   REPRESENTATIVE PUBLICATIONS: Brown KH, Drew RE, Weber LE, Thorgaard, GH. (2006). Intraspecific variation in the rainbow trout mitochondrial genome. Comp. Biochem. Physiol. D 1: 219-226. Sundin K., Brown KH, Drew RE, Nichols KM, Wheeler PA, Thorgaard GH. (2005) Genetic analysis of a development rate QTL in backcrosses of clonal rainbow trout. Aquaculture 247: 75-83. Felip A, Young WP, Wheeler PA, Thorgaard GH. (2005). An AFLP-based approach for the identification of sex-linked markers in rainbow trout (Oncorhynchus mykiss). Aquaculture 247: 35-43. Felip A, Fujiwara A, Young WP, Wheeler PA, Noakes M, Phillips RB, Thorgaard GH. (2004). Polymorphism and differentiation of rainbow trout Y chromosomes. Genome 47: 1105-1113. Nichols KM, Young WP, Danzmann RG, Robison BD, Rexroad C, Noakes M, Phillips RB, Bentzen P, Spies I, Knudsen K, Allendorf FW, Cunningham BM, Brunelli J, Zhang H, Ristow S, Drew R, Brown KH, Wheeler PA, Thorgaard GH. (2003) A consolidated linkage map for rainbow trout (Oncorhynchus mykiss). Anim Genet. 34(2):102-15. Palti, Y. , K.M. Nichols, K.I. Waller, J.E. Parsons and G.H. Thorgaard, 2001. Association between DNA polymorphisms tightly linked to MHC class II genes and IHN virus resistance in backcrosses of rainbow and cutthroat trout. Aquaculture 194: 283-289. Robison, B.D., P. A. Wheeler, K. Sundin, P. Sikka, and G. H. Thorgaard, 2001. Composite interval mapping reveals a major locus influencing embryonic development rate in rainbow trout (Oncorhynchus mykiss). J. Hered.: in press. Ostberg, C.O. and G.H. Thorgaard, 1999 Geographic distribution of chromosome and microsatellite DNA polymorphisms in Oncorhynchus mykiss native to western Washington. Copeia 1999: 287-298. Brunelli, J.P. and G.H. Thorgaard, 1999. Sequence, expression and genetic mapping of a rainbow trout retinoblastoma cDNA. Gene 226: 175-180. Palti, Y., J. E. Parsons and G. H. Thorgaard, 1999. Identification of candidate DNA markers associated with IHN virus resistance in backcrosses of rainbow (Oncorhynchus mykiss ) and cutthroat trout (O. clarki ). Aquaculture 173: 81-94. Robison, B.D., P.A. Wheeler and G.H. Thorgaard, 1999. Variation in development rate among clonal lines of rainbow trout (Oncorhynchus mykiss).Aquaculture 173: 131-141. Thorgaard, G.H., P.A.Wheeler, J.G. Cloud and T.R. Tiersch, 1998. Gene banking efforts for endangered fishes in the United States. In: Action before Extinction: An International Conference on Conservation of Fish Genetic Diversity. B. Harvey, C. Ross, D. Greer and J. Carolsfeld, eds. World Fisheries Trust, Victoria, British Columbia, pp. 181-185, Young, W.P., P.A. Wheeler, V.H. Coryell, P. Keim and G. H. Thorgaard,1998. A detailed linkage map of rainbow trout produced using doubled haploids. Genetics 148: 839-850. Galbreath, P.F. and G.H. Thorgaard, 1997. Saltwater performance of triploid Atlantic salmon Salmo salar L. X brown trout Salmo trutta L. hybrids. Aquaculture Research 28: 1-8. Young, W.P., P.A. Wheeler, R.D. Fields, and G.H. Thorgaard (1996). DNA fingerprinting confirms isogenicity of androgenetically-derived rainbow trout lines. J. Hered. 87:77-81. Spruell, P. and G.H. Thorgaard (1996). SINE sequences detect DNA fingerprints in salmonid fishes. Heredity 76: 317-324. Ristow, S.S., L.D. Grabowski, P.A. Wheeler, D.J. Prieur, and G.H. Thorgaard (1995). Arlee line of rainbow trout (Oncorhynchus mykiss) exhibits a low level of nonspecific cytotoxic cell activity. Dev. Comp. Immunol. 19: 497-505.   Young, W.P., P.A. Wheeler, and G.H. Thorgaard (1995). Asymmetry and variability of meristic characters and spotting in isogenic lines of rainbow trout. Aquaculture 137: 67-76.   Thorgaard, G.H., P. Spruell, P.A. Wheeler, P.D. Scheerer, A.S. Peek, J.J. Valentine, and B. Hilton (1995). Incidence of albinos as a monitor for induced triploidy in rainbow trout. Aquaculture 137: 121-130.   Galbreath, P.F and G.H. Thorgaard (1995). Sexual maturation and fertility of diploid and triploid Atlantic salmon X brown trout hybrids. Aquaculture 137: 299-311.   Galbreath, P.F. and G.H. Thorgaard (1995). Saltwater performance of all-female triploid Atlantic salmon. Aquaculture 138: 77-85.   Thorgaard, G.H., P. Spruell, S.A. Cummings, A.S. Peek, and E.L. Brannon (1995). Mixed DNA fingerprint analysis differentiates sockeye salmon populations. American Fisheries Society Symposium 17: 295-303.