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RESEARCH INTERESTS: Embryo and Sperm Physiology RESEARCH SUMMARY: Our laboratory currently has two areas of study. The first is a collaborative effort with Dr. Joanna Ellington (Co-PI NIH First Award) to establish and utilize oviductal cell lines (human, bovine, monkey and horse) with sperm cell culture systems for future diagnostic or therapeutic uses in the field of male factor infertility. An inherent component of this research is to advance understanding into the molecular mechanisms of sperm storage in females. The second area of study involves the optimization of bovine
in vitro fertilization through media components and cellular
co-culture systems. Much of this work involves the development
of non-invasive techniques to evaluate embryo quality.
REPRESENTATIVE PUBLICATIONS: Bosch P, de Avila JM, Ellington JE, Wright RW Jr. 2001 Heparin and Ca2+-free medium can enhance release of bull sperm attached to oviductal epithelial cell monolayers. Theriogenology. 56(2):247-60. Elias Calles JA, Gaskins CT, Busboom JR, Duckett SK, Cronrath JD, Reeves
JJ, Wright RW 2000. Differences among Wagyu sires for USDA carcass
traits and palatability attributes of cooked ribeye steaks. J Anim Sci.
78:1710-5. Ellington J.E., J. Samper, A. Jones, S.A. Oliver, K. Burnett,
R.W. Wright Jr. 1999. Effects of bovine serum albumin
on cryopreserved stallion sperm function during media cultures
and oviduct epithelial cell culture. Amer. J. Vet. Res. 60:363-367. Schneider C.S., J.E. Ellington, R.W. Wright Jr. 1999.
Effects of bulls with different field fertility on in vitro embryo
cleavage and development using sperm co-culture systems. Theriogenology
51:1085-1098. Ellington J.E., J. Samper, A.E. Jones, S.A. Oliver, K. Barnett,
R.W. Wright, Jr. 1999. Interactions of cryopreserved stallion
sperm and oviduct epithelial cells on their products in vitro.
Anim. Reprod. Sci 56:51-65. Ellington J.E., L. Broemeling, S. Broder, A.E. Jones, D.A.
Choker, R.W. Wright Jr. 1999. Kinetics of fresh and cryopreserved
human sperm attachment to oviduct (fallopian tubes) epithelial
cells in vitro. J. Andrology 20:492-499. Ellington J.E., D.P. Evenson, R.S. Brisbois, G.A. Hiss, A.E.
Jones, R.W. Wright Jr. 1999. The higher quality human
sperm in a sample selectively attach to oviduct (fallopian tube)
epithelial cells in vitro. Fert. Steril. 71:924-929. O'Fallon, J.V., R.W. Wright Jr., R.E. Calza. 1996.
Enzymatic Method for Measuring Subnanomolar Concentrations of
Oxygen in Gaseous or Aqueous Solutions. Anal. Biochem. Wright R.W. Jr., J. Ellington. 1995. Comparison of
in vivo- versus in vitro-derived preimplantation embryos from
the farm species. Theriogenology 44:1167-1189. Rehman N., A.R. Collins, T.K. Suh, R.W. Wright Jr.
1994. Development of in vitro-matured and fertilized bovine oocytes
matured in vitro. Theriogenology 41:1453-1462. Rehman N., A.R. Collins, T.K. Suh, R.W. Wright Jr.
1994. Effect of sperm exposure time on in vitro fertilization
and embryo development of bovine oocytes matured in vitro. Theriogenology
41:1447-1452. Rehman N., A.R. Collins, T.K. Suh, R.W. Wright Jr. 1994. Development of IVM- IVF-produced 8-cell bovine embryos in simple, serum-free media after conditioning or co-culture with Buffalo rat liver cells. Mol. Reprod. Develop. 38:251-255. |
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