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Research Interests
Migration and Invasion in Cancer and Cytotrophoblast Cell
Biology.
Research Summary
A primary focus of my laboratory is to determine, characterize and test the feasibility of cancer cell-specific targets and markers in order to detect and treat this disease. We have several promising leads in pursuit of this goal. The lethal phase of cancer is its spread to other parts of the body. This spread, or migration, has been attributed to integrins, receptor tyrosine kinase (RTKs) activation and their respective ligands. However, these proteins are not carcinoma cell specific targets because they are expressed in many normal tissues such as bone, intestine, bone marrow, spleen and muscle to maintain or repair physiology. Using a functional genomic approach, we have isolated several early genes induced by RTK activation of carcinoma cell migration. Currently, we are focusing on the most promising marker and target we have designed as Migration inducing gene 7 (Mig-7); expression that is induced by Hepatocyte growth factor also known as Scatter factor (HGF/SF), epidermal growth factor (EGF) or insulin like growth factor-1 (ILG-1) in concert with _ v_5 intergrin ligation. Mig-7 is expressed prior to and during cancer cell migration and invasion. Most importantly, we have not detected, even with RT-PCR (40 cycles) Mig-7 expression in normal tissue (n=6 each site) including brain, heart, kidney, spleen, liver, colon, lung, small intestine, muscle, stomach, testis, term placenta, salivary gland, thyroid, adrenal, pancreas, ovary, uterus, prostate, skin, peripheral blood leukocytes, bone marrow, fetal brain and fetal liver.
Research Publications
Gaasch, J.A. Bolwahnn, A.B. and Lindsey, J.S. (2006) Hepatocyte growth factor-regulated genes in differentiated RAW 264.7 osteoclast and undifferentiated cells. GENE 369:142-152
Phillips T.M. and Lindsey, J.S. (2005) Carcinoma Cell-specific Mig-7: A new potential maker for circulating and migrating cancer cells. Oncology Reports. 13(1):37-44.
Crouch S, Spidel CS, Lindsey JS. (2004) HGF and ligation of _v_5
integrin induce a novel, cancer cell-specific gene expression
required for cell scattering.
Exp Cell Res. 292(2):274-87.
Lindsey JS, Brenner RM. (2002) Novel hepatocyte growth factor/scatter factor isoform transcripts in the macaque endometrium and placenta. Mol Hum Reprod. 8(1):81-7.
K.A. Sutton, S. Maiti, W.A. Tribley, J.S. Lindsey, M.L. Meistrich, C.D. Bucana, B.M. Sanborn, D.R. Joseph, M.D. Griswold, G.A. Cornwall, M.F. Wilkinson. (1998) Androgen regulation of the Pem homeodomain gene in Sertoli and epididymal cells. Journal of Andrology 19(1):21-30.