Andrew G. McCubbin
Research SummaryMy lab focuses on plant preproduction, specifically intra-cellular signaling in pollen tube growth and self-incompatibility mechanisms in flowering plants. An array of self-incompatibility (S-I) mechanisms have evolved in plants that act to promote outbreeding and genetic diversity. We are studying the molecular basis of heteromorphic SI in Primula and Turnera species. In HSI systems floral morphology and a biochemical incompatibility system combine to both promote cross pollination and prevent self-fertilization. Our lab is using NexGen sequencing to sequence the multigene loci that control these systems, identify the pathways through which they act and then develop transgenic plants to test individual gene function.
A second avenue of research is intracellular signaling in pollen tube growth, currently the roles of calcium-dependent protein kinases in regulating growth polarity is under investigation. Lastly we are also part on a collaboration to develop a molecular diagnostic kit, which will allow farmers to assess wheat quality. Wheat can suffer from several problems that affect quality and value, two of these are Late Maturity Amylase (LMA) and Post Harvest Sprouting (PHS). LMA and PHS are both associated with loss of starch quality, but happen at different times in seed development. We are making antibodies to some of the enzymes associated with these phenomena to develop an immunochromatography assay (most familiar as to pregnancy test kits) to help farmers diagnose quality in the field and at grain silos.
Burrows, B. and McCubbin A.G. (2017) Reproduction overview by phylogeny: Plant. Ch. 20609. Encyclopedia of Reproduction, Elsvier (invited review chapter). https://doi.org/10.1016/B978-0-12-811899-3.20609-5
McCubbin A. (2017) Breeding barriers: Duplicate S genes form new barrier. Nature Plants, 26 June 2017 (invited “news and views” article).
Burrows, B. and McCubbin A.G. (2017). Sequencing the genomic regions flanking S-linked PvGLO sequences confirms the presence of two GLO loci, one of which lies adjacent to the style-length determinant gene CYP734A50. Plant Reprod. 2017 Mar;30(1):53-67. doi: 10.1007/s00497-017-0299-9.
Guo, F., Yoon G.M, and McCubbin, A.G. (2013) PiSCP1 and PiCDPK2 localize to peroxisomes and are involved in pollen tube growth in Petunia inflata. Plants 2, 72-86.
Guo F and McCubbin, A.G. (2012). The pollen specific R-SNARE/longin PiVAMP726 mediates fusion of endo- and exocytic compartments in pollen tube tip growth. J. Exp. Bot. 63: 3083-95.
McCubbin, A.G. (2008) Heteromorphic self-incompatibility in Primula: Twenty-first century tools promise to unravel a classic nineteenth century model system. In "Self-Incompatibility in Flowering Plants, evolution, diversity and mechanisms". Ed. V. Franklin-Tong. Springer. Pp 286-308.
McCubbin AG, Lee C, Hetrick A. 2006 Identification of genes showing differential expression between morphs in developing flowers of Primula vulgaris. Sexual Plant Repriduction: DOI: 10.1007/s00497-006-0022-8.
Yoon, G.M., P.E. Dowd, S. Gilroy, and A.G. McCubbin, Calcium-dependent protein kinase isoforms in Petunia have distinct functions in pollen tube growth, including regulating polarity. Plant Cell, 2006. 18(4): p. 867-78.
McCubbin, A.G. and E.H. Roalson, Construction of bacterial artificial chromosome libraries for use in phylogenetic studies. Methods Enzymol, 2005. 395: p. 384-400.
McCubbin, A.G., Ritchie, S.M., Swanson, S.J. and Gilroy, S. (2004) The calcium-dependent protein kinase HvCDPK1 mediates the gibberellic acid response of the barley aleurone through regulation of vacuolar function. Plant Journal 39:206-218.