|Department:||School of Molecular Biosciences, WSU|
|Credentials:||2002~Ph.D., Massachusetts Institute of Technology-Biology|
|Mailing Address:||School of Molecular Biosciences|
PO Box 647520
Pullman, WA 99164-7520
Genomics and Gene Expression
Cells respond to environmental or developmental signals by reprogramming the expression of specific genes throughout their genome. We wish to understand the mechanisms by which this occurs. To do so, we have used genomics-based approaches to decipher how gene expression is regulated in the model eukaryotes Saccharomyces cerevisiae and Arabidopsis thaliana, with the hope that the lessons we learn in yeast and plants will be applicable in mammalian systems. Ongoing projects include the (1) use of DNA microarrays to profile genome-wide mRNA levels in various transcription factor and histone mutant strains; (2) analysis of global protein-DNA interactions using DNA microarrays; and (3) the development of bioinformatics tools to analyze promoter sequences and DNA microarray data. These projects involve the development and use of cutting-edge biotechnology to investigate how genome expression is regulated in eukaryotic cells.
Chaudhuri, S., J.J. Wyrick, and M.J. Smerdon, Histone H3 Lys79 methylation is required for efficient nucleotide excision repair in a silenced locus of Saccharomyces cerevisiae. Nucleic Acids Res, 2009. 37(5): p. 1690-700.
Doroshenk, K.A., A.J. Crofts, R.T. Morris, J.J. Wyrick, and T. Okita, Proteomic Analysis of Cytoskeleton-Associated RNA Binding Proteins in Developing Rice Seed. J Res, 2009.
Jin, Y., A.M. Rodriguez, and J.J. Wyrick, Genetic and genomewide analysis of simultaneous mutations in acetylated and methylated lysine residues in histone H3 in Saccharomyces cerevisiae. Genetics, 2009. 181(2): p. 461-72.
Wyrick, J.J. and M.A. Parra, The role of histone H2A and H2B post-translational modifications in transcription: a genomic perspective. Biochim Biophys Acta, 2009. 1789(1): p. 37-44.
Morris, R.T., T.R. O'Connor, and J.J. Wyrick, Osiris: an integrated promoter database for Oryza sativa L. Bioinformatics, 2008.24(24): p. 2915-7.
Jin, Y., A.M. Rodriguez, J.D. Stanton, A.A. Kitazono, and J.J. Wyrick, Simultaneous mutation of methylated lysine residues in histone H3 causes enhanced gene silencing, cell cycle defects, and cell lethality in Saccharomyces cerevisiae. Mol Cell Biol, 2007. 27(19): p. 6832-41.
O'Connor, T.R. and J.J. Wyrick, ChromatinDB: a database of genome-wide histone modification patterns for Saccharomyces cerevisiae. Bioinformatics, 2007. 23(14): p. 1828-30.